Imaging and spectroscopic analysis of living cells. Imaging live cells in health and disease [electronic resource] / edited by P. Michael Conn.

Electronic reproduction. Amsterdam : Elsevier Science & Technology, 2012. Mode of access: World Wide Web. System requirements: Web browser. Title from title screen (viewed on Apr. 4, 2012). Access may be restricted to users at subscribing institutions.

This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. The second of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Part A: Viruses, Microbes and Parasites Nanoscale imaging of microbial pathogens using atomic force microscopy Challenges in imaging parasites Part B: Disease States Real-time live imaging to study bacterial infections in vivo Analysis of cellular membrane dynamics by following virus endocytosis Imaging of live malaria blood stage parasites Escherichia coli K1 invasion of human brain microvascular endothelial cells Real-time imaging after cerebral ischemia Imaging acute inflammation in zebrafish Imaging protein oligomerization in neurodegeneration using BiEC Imagng and quantifying the dyanmics of tumor-associated proteolysis Part C: Other Techniques Live cell imaging in vivo with fluorescent proteins Visualization of gene expression Imaging stem cell implant for cellular-based therapies Live cell single molecule imaging to investigate early events in B lymphocyte signaling A quantitative method for measuring phototoxicity of a live cell imaging microscope High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines High-content screening of primary neurons Live-imaging fluorescent proteins in early mouse embryos Comparing the methods for quantitative analysis of dendritic spine dynamics Live-imaging of cell competition in drosophila imaginal epithelium Live cell imaging of the oviduct Computational estimation of leukocyte numbers in zebrafish Four dimensional tracking of lymphocyte migration and interactions by two-photon microscopy.

Includes bibliographical references and indexes.

Section 1. Viruses, microbes, and parasites. Microbial cells analysis by atomic force microscopy / David Alsteens ; Understanding parasite transmission through imaging approaches / Mirko Singer and Freddy Frischknecht ; Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo / Ferdinand X. Choong ... [et al.] ; Analysis of virus entry and cellular membrane dynamics by single particle tracking / Helge Ewers and Mario Schelhaas ; Imaging of live malaria blood stage parasites / Christof Gr�uring and Tobias Spielmann ; Escherichia coli K1 invasion of human brain microvascular endothelial cells / Lip Nam Loh and Theresa H. Ward -- Section 2. Disease states. Real-time imaging after cerebral ischemia : model systems for visualization of inflammation and neuronal repair / Pierre Cordeau and Jasna Kriz ; In vivo real-time visualization of leukocytes and intracellular hydrogen peroxide levels during a zebrafish acute inflammation assay / Luke Pase, Cameron J. Nowell, and Graham J. Lieschke ; Imaging protein oligomerization in neurodegeneration using bimolecular fluorescence complementation / Federico Herrera, Susana Gon�calves, and Tiago Fleming Outeiro ; 3D/4D functional imaging of tumor-associated proteolysis : impact of microenvironment / Kamiar Moin ... [et al.] -- Section 3. Other techniques. Live cell imaging in live animals with fluorescent proteins / Robert M. Hoffman ; Protein activation dynamics in cells and tumor micro arrays assessed by time resolved F�orster resonance energy transfer / V�eronique Calleja, Pierre Leboucher, Banafshe� Larijani ; Imaging stem cell differentiation for cell-based tissue repair / Zhenghong Lee ... [et al.] ; Understanding the initiation of B cell signaling through live cell imaging / Angel M. Davey ... [et al.] ; A quantitative method for measuring phototoxicity of a live cell imaging microscope / Jean-Yves Tinevez ... [et al.] ; High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines / Davide Danovi ... [et al.] ; High-throughput screening in primary neurons / Punita Sharma ... [et al.] ; Live imaging fluorescent proteins in early mouse embryos / Panagiotis Xenopoulos, Sonja Nowotschin, and Anna-Katerina Hadjantonakis ; Methods for three-dimensional analysis of dendritic spine dynamics / Enni Bertling ... [et al.] ; Imaging cell competition in Drosophila imaginal discs / Shizue Ohsawa ... [et al.] ; Live cell imaging of the oviduct / Sabine K�olle -- Computational quantification of fluorescent leukocyte numbers in zebrafish embryos / Felix Ellett and Graham J. Lieschke -- Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy / Masahiro Kitano and Takaharu Okada.