Guide to yeast genetics and molecular biology [electronic resource] / edited by Christine Guthrie, Gerald R. Fink.

Contributor(s): Guthrie, Christine | Fink, Gerald RMaterial type: Text

TextSeries: Methods in enzymology ; v. 194.Publication details: San Diego : Academic Press, c1991. Description: 1 online resource (xxxvii, 933 p.)ISBN: 0121820955 (electronic bk.); 9780121820954 (electronic bk.)Subject(s): Yeast fungi -- Genetics | Yeast fungi -- Cytology | Yeast fungi -- Cytology | Yeast fungi -- GeneticsGenre/Form: Electronic books.DDC classification: 589.233 LOC classification: QP601Online resources: ScienceDirect | ScienceDirect

Partial contents:

Getting started with yeast / Fred Sherman -- Micromanipulation and dissection of asci / Fred Sherman, James Hicks -- Mapping yeast genes / Fred Sherman, Paul Wakem -- Positional mapping of genes by chromosome blotting and chromosome fragmentation / Sandra L. Gerring, Carla Connelly, Philip Hieter -- Assay of yeast mating reaction / George F. Sprague, Jr. -- Monitoring meiosis and sporulation in Saccharomyces cerevisiae / Yona Kassir, Giora Simchen -- Identifying sporulation genes, visualizing synaptonemal complexes, and large-scale spore and spore wall purification / Rochelle Easton Esposito, Michael Dresser, Michael Breitenbach -- Putting the HO gene to work : practical uses for mating-type switching / Ira Herskowitz, Robert E. Jensen -- Spore enrichment / Beth Rockmill, Eric J. Lambie, G. Shirleen Roeder -- Analysis and manipulation of yeast mitochondrial genes / Thomas D. Fox ... [et al.] -- DNA of Saccharomyces cerevisiae / Peter Philippsen, Agathe Stotz, Christine Scherf -- High-efficiency transformation of yeast by electroporation / Daniel M. Becker, Leonard Guarente -- Transmission of plasmid DNA to yeast by conjugation with bacteria / Jack A. Heinemann, George F. Sprague, Jr. -- Cloning genes by complementation in yeast / Mark D. Rose, James R. Broach -- Gene isolation with [lambda]gt11 system / Richard A. Young, Ronald W. Davis -- Gene overexpression in studies of Saccharomyces cerevisiae / Jasper Rine -- Preparation of clone libraries in yeast artificial-chromosome vectors / David T. Burke, Maynard V. Olson -- Classical mutagenesis techniques / Christopher W. Lawrence -- Targeting, disruption, replacement, and allele rescue : integrative DNA transformation in yeast / Rodney Rothstein -- In vitro mutagenesis and plasmid shuffling : from cloned gene to mutant yeast / Robert S. Sikorski, Jef D. Boeke -- Recovery of plasmids from yeast into Escherichia coli : shuttle vectors / Jeffrey N. Strathern, David R. Higgins -- Shuttle mutagenesis : bacterial transposons for genetic manipulations in yeast / Merl F. Hoekstra ... [et al.] -- Ty mutagenesis in Saccharomyces cerevisiae / David J. Garfinkel, Jeffrey N. Strathern -- Transformation of yeast directly with synthetic oligonucleotides / Richard P. Moerschell, Goutam Das, Fred Sherman -- Vectors for expression of cloned genes in yeast : regulation, overproduction, and underproduction / Jane C. Schneider, Leonard Guarente -- Vectors for constitutive and inducible gene expression in yeast / Mark Schena, Didier Picard, Keith R. Yamamoto -- Preparation of high molecular weight RNA / Karl K�ohrer, Horst Domdey -- Preparation and analysis of low molecular weight RNAs and small ribonucleoproteins / Jo Ann Wise -- Measurement of mRNA decay rates in Saccharomyces cerevisiae / by Roy Parker ... [et al.] --Labeling of RNA and phosphoproteins in Saccharomyces cerevisiae / Jonathan R. Warner -- Tackling the protease problem in Saccharomyces cerevisiae / Elizabeth W. Jones -- Structural and functional analysis of yeast ribosomal proteins / H.A. Rau�e, W.H. Mager, R.J. Planta.

Summary: Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein.

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Includes indexes.

Includes bibliographical references.

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Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein.

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