Cellulases [electronic resource] / edited by Harry J. Gilbert.

Includes bibliographical references and indexes.

Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume in the Methods in Enzymology series comprehensively covers this topic.

Measurement of endo-1,4-[beta]-Glucanase -- Biomass Conversion Determined via Fluorescent Cellulose Decay Assay -- The Analysis of Saccharification in Biomass Using an Automated High-Throughput Method -- Studies of Enzymatic Cleavage of Cellulose Using Polysaccharide Analysis by Carbohydrate -- Measuring Processivity -- Distinguishing Xyloglucanase Activity in endo-[beta](1-->4)glucanases -- Methods for Structural Characterization of the Products of Cellulose- and Xyloglucan-Hydrolyzing Enzymes -- The Crystallization and Structural Analysis of Cellulases (and Other Glycoside Hydrolases) -- Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose -- Small-Angle X-ray Scattering and Crystallography -- Quantitative Approaches to The Analysis of Carbohydrate-Binding Module Function -- In Situ Detection of Cellulose with Carbohydrate-Binding Modules -- Interactions Between Family 3 Carbohydrate Binding Modules (CBMs) and Cellulosomal Linker Peptides -- Approaches for Improving Thermostability Characteristics in Cellulases -- Thermophilic Glycosynthases for Oligosaccharides Synthesis -- Engineering Cellulase Activity into Clostridium acetobutylicum -- Transformation of Clostridium Thermocellum by Electroporation -- Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus -- Methods for the Isolation of Cellulose-Degrading Microorganisms -- Metagenomic Approaches to the Discovery of Cellulases -- Escherichia coli Expression, Purification, Crystallization, and Structure Determination of Bacterial -- Measurements of Relative Binding of Cohesin and Dockerin Mutants Using an Advanced ELISA Technique -- Designer Cellulosomes for Enhanced Hydrolysis of Cellulosic Substrates -- High-Throughput Screening of Cohesin Mutant Libraries on Cellulose Microarrays.