Advanced bacterial genetics [electronic resource] : use of transposons and phage for genomic engineering / edited by Kelly T. Hughes, Stanley R. Maloy.
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TextSeries: Methods in enzymology ; v. 421.Publication details: Amsterdam ; Boston : Elsevier / Academic Press, c2007. Description: 1 online resource (xxxiv, 284 p.) : illISBN: 9780080475103 (electronic bk.); 0080475108 (electronic bk.); 0123737494 (electronic bk.); 9780123737496 (electronic bk.)Subject(s): Bacterial genetics | Transposons | Bacteriophages | SCIENCE -- Life Sciences -- Biochemistry | Genetische manipulatie | Bacteri�en | Transposonen | Bacteriofagen | Genoom | Genomica | Mutagenesis | Bacterial genetics | Bacteriophages | Transposons | Bacteria -- genetics | DNA, BacterialGenre/Form: Electronic books.Additional physical formats: Print version:: Advanced bacterial geneticsDDC classification: 572/.7 LOC classification: QP601 | .M49 vol. 421NLM classification: W1 | QW 51Other classification: 42.33 | 35.71 Online resources: ScienceDirect Includes bibliographical references and indexes.
Strain collections and genetic nomenclature / Stanley R. Maloy and Kelly T. Hughes -- Use of antibiotic-resistant transposons for mutagenesis / Stanley R. Maloy -- In vivo mutagenesis using EZ-Tn5 / John R. Kirby -- Identification of essential genes in bacteria / David R. Hillyard and Michael J. Reed -- Isolation of transposon insertions / Stanley R. Maloy -- Localized mutagenesis / Stanley R. Maloy -- Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / Kelly T. Hughes -- Target-directed proteolysis in vivo / Markus Eser ... [et al.] -- Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / Beth Traxler and Eliora Gachelet -- Using genomic microarrays to study insertional/transposon mutant libraries / David N. Baldwin and Nina R. Salama -- Screening transposon mutant libraries using full-genome oligonucleotide microarrays / Kelly M. Winterberg and William S. Reznikoff -- Creating recombination-activated genes and sequence-defined mutant libraries using transposons / Larry Gallagher ... [et al.] -- Use of operon and gene fusions to study gene regulation in Salmonella / Kelly T. Hughes and Stanley R. Maloy -- Genomic screening for regulatory genes using the T-POP transposon / Changhan Lee ... [et al.] -- Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / James A. Sawitzke ... [et al.] -- [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / Joyce E. Karlinsey -- Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / Nathalie Garcia-Russell, Samantha S. Orchard, and Anca M. Segall -- Dissecting nucleic acid-protein interactions using challenge phage / Stanley R. Maloy and Jeffrey Gardner -- Mud-P22 / Jenny A. Crain and Stanley R. Maloy -- Phage metagenomics / Veronica Casas and Forest Rowher.
Description based on print version record.
A volume of indispensable methods for the use of transposons and phage in genomic engineering.
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