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Digital microscopy [electronic resource] : a second edition of Video microscopy / edited by Greenfield Sluder, David E. Wolf.

Contributor(s): Wolf, D. E. (David E.) | American Society for Cell BiologyMaterial type: TextTextSeries: Methods in cell biology ; v. 72.Publication details: Amsterdam ; Boston : Elsevier Academic Press, �2003. Edition: 2nd edDescription: 1 online resource (xviii, 523 pages, [19] pages of plates) : illustrations (some color)Content type: text Media type: computer Carrier type: online resourceISBN: 9780080546032 (electronic bk.); 008054603X (electronic bk.); 0125641699 (electronic bk.); 9780125641692 (electronic bk.)Subject(s): Microscopy | Image processing -- Digital techniques | Video microscopy | Image Processing, Computer-Assisted -- instrumentation | Image Processing, Computer-Assisted -- methods | Microscopy -- instrumentation | Microscopy -- methods | NATURE -- Reference | SCIENCE -- Life Sciences -- General | SCIENCE -- Life Sciences -- Biology | Image processing -- Digital techniques | Microscopy | Video microscopy | Microscopie | Digitale techniekenGenre/Form: Electronic books.Additional physical formats: Print version:: Digital microscopy.DDC classification: 570.282 LOC classification: QH585 | .M38 2003ebNLM classification: W1 | QH 207Online resources: ScienceDirect
Contents:
Chapter 1. Microscope Basics -- Chapter 2. Optics of The Microscope Image Formation -- Chapter 3. Proper Alignment of the Microscope -- Chapter 4. Mating Cameras to Microscopes --Chapter 5. ''Do Not (Mis- ) Adjust Your Set'': Maintaining Specimen Detail in the Video Microscope -- Chapter 6. Cameras for Digital Microscopy -- Chapter 7. Electronic Cameras for Low-Light Microscopy -- Chapter 8. Cooled vs. Intensified vs. Electron Bombardment CCD Cameras-Applications and Relative Advantages -- Chapter 9. Fundamentals of Fluorescence and Fluorescence Microscopy -- Chapter 10. A High-Resolution Multimode Digital Microscope System -- Chapter 11. Fundamentals of Image Processing in Light Microscopy -- Chapter 12. Techniques for Optimizing Microscopy and Analysis through Digital Image Processing -- Chapter 13. The Use and Manipulation of Digital Image Files in Light Microscopy -- Chapter 14. High-Resolution Video-Enhanced Differential Interference Contrast Light Microscopy -- Chapter 15. Quantitative Digital and Video Microscopy -- Chapter 16. Computational Restoration of Fluorescence Images: Noise Reduction, Deconvolution, and Pattern Recognition -- Chapter 17. Quantitative Fluorescence Microscopy and Image Deconvolution -- Chapter 18. Ratio Imaging: Measuring Intracellular Ca++ and pH in Living Cells -- Chapter 19. Ratio Imaging Instrumentation -- Chapter 20. Fluorescence Resonance Energy Transfer Microscopy: Theory and Instrumentation -- Chapter 21. Fluorescence-Lifetime Imaging Techniques for Microscopy -- Chapter 22. Fluorescence Correlation Spectroscopy: Molecular Complexing in Solution and in Living Cells.
Summary: The text is richly illustrated, lightly written and more wide-ranging than Volume 1. A comprehensive treatment of fluid-structure interactions involving axial flow and slender structures, such as piping, human veins, aircraft, nuclear reactor fuel and submarine skins. The emphasis is on fundamentals, particularly on the physical understanding and underlying mechanisms, as well as on applications.
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Includes bibliographical references and index.

Chapter 1. Microscope Basics -- Chapter 2. Optics of The Microscope Image Formation -- Chapter 3. Proper Alignment of the Microscope -- Chapter 4. Mating Cameras to Microscopes --Chapter 5. ''Do Not (Mis- ) Adjust Your Set'': Maintaining Specimen Detail in the Video Microscope -- Chapter 6. Cameras for Digital Microscopy -- Chapter 7. Electronic Cameras for Low-Light Microscopy -- Chapter 8. Cooled vs. Intensified vs. Electron Bombardment CCD Cameras-Applications and Relative Advantages -- Chapter 9. Fundamentals of Fluorescence and Fluorescence Microscopy -- Chapter 10. A High-Resolution Multimode Digital Microscope System -- Chapter 11. Fundamentals of Image Processing in Light Microscopy -- Chapter 12. Techniques for Optimizing Microscopy and Analysis through Digital Image Processing -- Chapter 13. The Use and Manipulation of Digital Image Files in Light Microscopy -- Chapter 14. High-Resolution Video-Enhanced Differential Interference Contrast Light Microscopy -- Chapter 15. Quantitative Digital and Video Microscopy -- Chapter 16. Computational Restoration of Fluorescence Images: Noise Reduction, Deconvolution, and Pattern Recognition -- Chapter 17. Quantitative Fluorescence Microscopy and Image Deconvolution -- Chapter 18. Ratio Imaging: Measuring Intracellular Ca++ and pH in Living Cells -- Chapter 19. Ratio Imaging Instrumentation -- Chapter 20. Fluorescence Resonance Energy Transfer Microscopy: Theory and Instrumentation -- Chapter 21. Fluorescence-Lifetime Imaging Techniques for Microscopy -- Chapter 22. Fluorescence Correlation Spectroscopy: Molecular Complexing in Solution and in Living Cells.

The text is richly illustrated, lightly written and more wide-ranging than Volume 1. A comprehensive treatment of fluid-structure interactions involving axial flow and slender structures, such as piping, human veins, aircraft, nuclear reactor fuel and submarine skins. The emphasis is on fundamentals, particularly on the physical understanding and underlying mechanisms, as well as on applications.

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