Mitochondria [electronic resource] / edited by Liza A. Pon, Eric A. Schon.

"Prepared under the auspices of the American Society for Cell Biology."

Includes bibliographical references and index.

Isolation and subfractionation of mitochondria from animal cells and tissue culture lines -- Isolation and subfractionation of mitochondria from the yeast Saccharomyces cerevisiae -- Isolation and subfractionation of mitochondria from plants -- Assessing functional integrity of mitochondria in vitro and in vivo -- Assaying mitochondrial respiratory complex activity in mitochondria isolated from human cells and tissues -- In vivo measurements of respiration control by cytochrome c oxidase and in situ analysis of oxidative phosphorylation -- Assay of mitochondrial ATP synthesis in animal cells -- Measurement of membrane permeability and permeability transition of mitochondria -- Assaying actin-binding activity of mitochondria in yeast -- Analysis and prediction of mitochondrial targeting peptides -- Assaying protein import into mitochondria -- Analysis of protein-protein interactions in mitochondria by coimmunoprecipitation and chemical cross-linking -- Blue-native gels to isolate protein complexes from mitochondria -- Application of electron tomography to mitochondrial research -- Epitope tagging and visualization of nuclear-encoded mitochondrial proteins in yeast -- Targeting of green fluorescent protein to mitochondria -- Assessment of mitochondrial membrane potential in situ using single potentiometric dyes and a novel fluorescence resonance energy transfer technique -- Optical imaging techniques (histochemical, immunohistochemical, and in situ hybridization staining methods) to visualize mitochondria -- Visualization of mitochondrial movement in yeast -- Targeting of reporter molecules to mitochondria to measure calcium, ATP, and pH -- Genetic transformation of Saccharomyces cerevisiae mitochondria -- Transmitochondrial technology in animal cells -- Diagnostic assays for defects in mitochondrial DNA replication and transcription in yeast and human cells -- Analysis of mitochondrial translation products in Vivo and in organello in yeast -- Numerical methods for handling uncertainty in microarray data: An example analyzing perturbed mitochondrial function in yeast.

This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies.

Description based on print version record.