Microtubules, in vitro [electronic resource] / edited by Leslie Wilson, John J. Correia.

Includes bibliographical references and index.

Section I. Isolation and biochemistry of tubulin and characterization of antibodies and isotypes.-- Preparation of microtubule protein and purified tupbulin from bovine brain by cycles of assembly and disassembly and phosphocellulose chromatography / Herbert P. Miller, Leslie Wilson.-- Isolating tubulin from nonneural sources / Dan L. Sackett, Karl A. Werbovetz, Naomi S. Morrissette.-- Characterization of anti-B-tubulin antibodies / Anthony J. Spano, Anthony Frankfurter.-- Expression profliing of tubulin isotypes and microtubule-interacting proteins using real-time polymerase chain reaction / Sharon Lobert, Laree Hiser, John J. Correia.-- Nondenaturing electrophoresis as a tool to investigate tabulin complexes / Monica Lopez Fanarago, Gerardo Carranza, Raquel Castano, Sofia Nolasco, J. Avila, J.C. Zabala.-- Mass spectrometry analysis of c-terminal postranslational modifications of tubulins / Virginie Redeker.-- Methods in tubulin proteomics / Leah M. Miller, Hui Xiao, Berta Burd, Susan Band Horwitz, Ruth Hogue Angeletti, Pascal Verdier-Pinard.-- Section II. Microtubule structure and dynamics.-- Cryo-em studies of microtubule structural intermediates and kinetochore-microtubule interactions / Eva Nogales, Vincent H. Rame, Hong-Wei Wang.-- High-resolution imaging of microtubules and cytoskeleton structures by atomic force microscopy / Loic Hamon, Patrick A. Curmi, David Patre.-- Using computational modeling to understand microtubule dynamics : a primer for cell biologists.-- Analysis of dynamic instability of steady-state microtubules in vitro by video-enchanced differential interference contrast microscopy with an appendix by Emin Oroudjev / Mythili Yenjerla, Manu Lopus, Leslie Wilson.-- Nanometer-resolution microtubule polymerization assays using optical tweezers and microfabricated barriers/ Blake d. Charlebois, Henry T. Schek III, Alan J. Hunt.-- Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy / Christopher Gell, Volker Bormuth, Gary J. Bronhard, Daniel N. Cohen, Stefan Diez, Claire T. Friel, Jonne Helenius, Bert Nitzsche, Heike Petzold, Jan Ribbe, Erik Schiffer, Jeffrey H. Stear, Anastasiya Trushko, Vladimir Varga, Per O. Widlund, Marija Zanic, Jonathan Howard.-- Studying kinesin motors by optical 3D-nanometry in gliding motility assays / Bert Nitzsche, Volker Bormuth, Crina Brauer, Jonathan Howard Leonid Ionov, Jacob Kerssemakers, Till Korten, Cecile Leduc, Felix Ruhnow, Stefan Diez.-- Section III. Drugs.-- Analysis of tubulin oligomers by analytical ultricentrifugation / John J. Correia.-- Determination of drug binding to mactrotubules in vitro / Jennifer A. Smith, Mary Ann Jordan.-- Fluorescence spectroscopic methods to analyze drug-tubulin interactoins / Bhabatarak Bhattacharyya, Sonia Kapoor, Dulal Panda.-- Tubulin polymerizatin microassay used to compare ligand eficacy / Ashley Davis, Sarah Martinez, Daniel Nelson, Kim Middleton.-- Fluorescent taxoid probes for microtubule research / Isabel Barasoain, J. Fernando Diaz, Jose M. Andrea.-- Binding of vinca domain agents to tubulin: structural and biomedical studies / Anthony Cormier, Marcel Knossow, Chunguang Wang, Benoit Gigant.-- Measurement of ligand binding to tubulin by sulfhydryl reactivity / Adrian Begaye, Dan L. Sackett.-- Section IV. Interactions with motors and MAPs.-- Probing interactions of tubulin with small molecules, peptides, and protein fragments by solution nuclear magnetic resonance / Marie-Jeanne Clement, Philippe Savarin, Elisabeth Adjadj, Andre Sobel, Flavio Toma, Patrick A. Curmi.-- Microtubule and MAPs: thermodynamics of complex formation by AUC, ITC, fluorescence, and NMR / Francois Devred, Pascale Barbier, Daniel Lafitte, Isabelle Landrieu, Guy Lippens, Vincent Peyrot.-- Quantitative analysis of MAP-mediated regulation of microtubule dynamic instability in vitro--focus on tau / Erkan Kiris, Donovan Ventimiglia, Stuart C. Feinstein.-- Structure and dynamics of the kinesin-Microtubule interaction revealed by fluorescence polarization microscopy / Hernando Sosa, Ana B. Asenjo, Erwin J.G. Peterman.-- Multiple collor single molecule TIRF imaging and tracking of MAPs and motors / Jennifer L. Ross, Ram Dixit.-- Studying plus-end tracking at single molecule resolution using TIRF microscopy / Ram Dixit, Jennifer L. Ross.-- Fluorescence microscopy assays on chemically functionalized surfaces for quantitative imaging of microtubule, motor, and +TIP dynamics / Peter Bieling, Ivo A. Telley, Christian Hentrich, Jacob Piehler, Thomas Surrey.-- Section V. Functional extracts and force measurements.-- Quantitative characterization of filament dynamics by single-molecule lifetime measurements / Leonid A. Mirny, Daniel J. Needleman.-- Extracting the mechanical properties of microtubules from thermal fluctuation measurements on an attached tracer particle / Katja M. Taute, Francesco Pampaloni, Ernest-Ludwig Florin.-- In vitro assays to study force generation at dynamic microtubule ends / Liedewij Laan, Marileen Dogterom.-- Reconstitution and functional analysis of kinetochore subcomplexes / Daniel R. Gestaut, Jeremy Cooper, Charles L. Asbury, Trisha N. Davis, Linda Wordeman.-- In vitro assays to study the tracking of shortening microtubule ends and to measure associated forces / Ekaterina L. Grishchuk, Fazly I. Ataullakhanov.

There continues to be intense interest in the microtubule cytoskeleton, the assembly, structure and regulation of microtubules and the numerous motors and accessory proteins that control cell cycle, dynamics, organization and transport. The field continues to grow and explore new aspects of these issues driven immensely by developments in optical imaging and tracking techniques. This�volume (complimented by the forthcoming companion volume by Cassimeris and Tran) brings together current research and protocols in the field of microtoublues in vitro and will�serve as a valuable�to.

Print version record.