Cilia [electronic resource] : structure and motility / edited by Stephen M. King, Gregory J. Pazour.

Includes bibliographical references and index.

Title from PDF t.p. (Science Direct, viewed Mar. 9, 2010).

Cryo-electron microscope tomography to study axonemal organization / Daniela Nicastro -- Electron microscopic imaging and analysis of isolated dynein particles / Antony J. Roberts, Stan A. Burgess -- Immunogold labeling of flagellar components in situ / Stefan Geimer -- Scanning electron microscopy to examine cells and organs / Jovenal T. SanAgustin, John A. Follit, Gregory Hendricks, Gregory J. Pazour -- X-ray fiber diffraction studies on flagellar axonemes / Kazuhiro Oiwa, Shinji Kamimura, Hiroyuki Iwamoto -- Markers for neuronal cilia / Jacqueline S. Domire, Kirk Mykytyn -- Immunofluorescence staining of cliated respiratory epithelial cells / Heymut Omran, Niki T. Loges -- Immunoprecipitation to examine protein complexes / Gregory J. Pazour -- Tandem affinity prification of ciliopathy-associated protein complexes / Karsten Boldt, Jeroen van Reeuwijk, Christian Johannes Gloeckner, Marius Ueffing, Ronald Roepman -- Crosslinking methods: purification and analysis of crosslinked dynein products / Miho Sakato -- Analysis of ciliary/flagellar beating of Chlamydomonas / Kenneth W. Foster -- Assays of cell and axonemal motility in Chlamydomonas reinhardtii / Ritsu Kamiya -- High-speed digital imaging ependymal cilia in the murine brain / Karl-Ferdinand Lechtreck, Michael J. Sanderson, George B. Witman -- Observation of nodal cilia movement and measurement of nodal flow / Yasushi Ikada, Nobutaka Hirokawa -- Modification of mouse nodal flow by applying artificial flow / Shigenori Nonaka -- Measuring clium-induced Ca2+ increases in cultured renal epithelia / Helle A. Praetorius.

Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures.