Cellular electron microscopy [electronic resource] / edited by J. Richard McIntosh.

Includes bibliographical references and index.

The Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells / J. Dubochet -- Cryopreparation Methods for Electron Microscopy of Selected Model Systems / K. McDonald -- Cryopreparation Methodology for Plant Cell Biology / M.W. Hess -- Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis / T. Muller-Reichert -- Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography / E.T. O'Toole -- Whole-Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography / J.L. Hoog -- Electron Microscopy of Archaea / C.E. Robertson -- Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography / B.J. Marsh -- Visualization of Membrane-Cytoskeletal Interactions During Plant Cytokinesis / M.S. Otegui -- Electron Tomographic Methods for Studying the Chemical Synapse / C.P. Arthur -- Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle / B.F. McEwen -- Electron Microscopic Analysis of the Leading Edge in Migrating Cells / T. Svitkina -- Imaging Actomyosin In Situ / K.A. Taylor -- Electron Tomography of Bacterial Chemotaxis Receptor Assemblies / P. Zhang -- How to "Read" a Vitreous Section / J. Dubochet -- Single-Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction / I.I. Serysheva -- Electron Microscopy of Microtubule-Based Cytoskeletal Machinery / A. Hoenger -- Reconstructing the Endocytotic Machinery / Y. Cheng -- 3D Immunolocalization with Plastic Sections / M.K. Morphew -- Electron Microscopy Analysis of Viral Morphogenesis / A. Pelchen-Matthews -- Electron Tomography of Immunolabeled Cryosections / M.S. Ladinsky -- Visualizing Macromolecules with Fluoronanogold: From Photon Microscopy to Electron Tomography / T. Cheutin -- Markers for Correlated Light and Electron Microscopy / G.E. Sosinsky -- Localizing Specific Elements Bound to Macromolecules by EFTEM / R.D. Leapman -- Localization of Protein Complexes by Pattern Recognition / C. Best -- The Application of Energy-Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples / J.C. Bouwer -- Optimization of Image Collection for Cellular Electron Microscopy / P. Mooney -- Future Directions for Camera Systems in Electron Microscopy / N. Xuong -- Structure Determination In Situ by Averaging of Tomograms / F. Forster -- Methods for Image Segmentation in Cellular Tomography / K. Sandberg -- Database Resources for Cellular Electron Microscopy / M.E. Martone.

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, a.

Description based on print version record.