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Guide to protein purification (Record no. 21346)

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000 -LEADER
fixed length control field	06980cam a2200565Ia 4500
001 - CONTROL NUMBER
control field	ocn463300660
003 - CONTROL NUMBER IDENTIFIER
control field	OCoLC
005 - DATE AND TIME OF LATEST TRANSACTION
control field	20141103172217.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
fixed length control field	m o d
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field	cr mnu\|\|\|\|\|\|\|\|
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field	091104s2009 caua ob 001 0 eng
040 ## - CATALOGING SOURCE
Original cataloging agency
Language of cataloging	eng
Transcribing agency	UMC
Modifying agency	SCB
--	OCM
--	BTCTA
--	YDXCP
--	UKMGB
--	CUS
--	CRU
--	CDX
--	E7B
--	OCLCQ
--	OPELS
--	OCLCQ
--	OPELS
--	OCLCF
--	KUK
019 ## -
--	318877741
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number	9780123745361 (electronic bk.)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number	0123745365 (electronic bk.)
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
Canceled/invalid ISBN	9780123749789 (pbk.)
029 1# - (OCLC)
OCLC library identifier	DEBBG
System control number	BV040084208
035 ## - SYSTEM CONTROL NUMBER
System control number	(OCoLC)463300660
Canceled/invalid control number	(OCoLC)318877741
050 #4 - LIBRARY OF CONGRESS CALL NUMBER
Classification number	QP601
Item number	.M49 v. 463
060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER
Classification number	W1
Item number	ME9615K v.463 2009
060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER
Classification number	QU 55
Item number	G9446 2009
070 ## - NATIONAL AGRICULTURAL LIBRARY CALL NUMBER
Classification number	QP601
Item number	.G85 2009
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number	547.7
Edition number	22
049 ## - LOCAL HOLDINGS (OCLC)
Holding library	TEFA
245 00 - TITLE STATEMENT
Title	Guide to protein purification
Medium	[electronic resource] /
Statement of responsibility, etc.	edited by Richard R. Burgess, Murray P. Deutscher.
250 ## - EDITION STATEMENT
Edition statement	2nd ed.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc.	San Diego, Calif :
Name of publisher, distributor, etc.	Academic Press/Elsevier,
Date of publication, distribution, etc.	c2009.
300 ## - PHYSICAL DESCRIPTION
Extent	1 online resource (liii, 851 p.) :
Other physical details	ill.
490 1# - SERIES STATEMENT
Series statement	Methods in enzymology,
International Standard Serial Number	0076-6879 ;
Volume/sequential designation	v. 463
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc	Includes bibliographical references and indexes.
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note	Why purify enzymes? / A. Kornberg -- Strategies and considerations for protein purifications / S. Linn -- Use of bioinformatics in planning a protein purification / R.R. Burgess -- Preparing a purification summary table / R.R. Burgess -- Setting up a laboratory / M.P. Deutscher -- Buffers: principles and practice / V.S. Stoll and J.S. Blanchard -- Measurement of enzyme activity / T.K. Harris and M.M. Keshwani -- Quantitation of protein / J.E. Noble and M.J. Bailey -- Concentration of proteins and removal of solutes / D.R. Evans, J.K. Romero and M. Westoby -- Maintaining protein stability / M.P. Deutscher -- Selecting an appropriate method for expressing a recombinant protein / W.H. Brondyk -- Bacterial systems for production of heterologous proteins / S. Zerbs, A.M. Frank and F.R. Collart -- Expression in the yeast Pichia pastoris / J.M. Cregg ... [et al.] -- Baculovirus-insect cell expression systems / D.L. Jarvis -- Recombinant protein production by transient gene transfer into Mammalian cells / S. Geisse and C. Fux -- Tagging for protein expression / A. Malhotra -- Refolding solubilized inclusion body proteins / R.R. Burgess -- Advances in preparation of biological extracts for protein purification / A.C. Grabski -- Isolation of subcellular organelles and structures / U. Michelsen and J. von Hagen -- Protein precipitation techniques / R.R. Burgess -- Affi-Gel Blue for nucleic acid removal and early enrichment of nucleotide binding proteins / M.P. Deutscher -- Ion-exchange chromatography / A. Jungbauer and R. Hahn -- Gel filtration / E. Stellwagen -- Protein chromatography on hydroxyapatite columns / L.J. Cummings, M.A. Snyder and K. Brisack -- Theory and use of hydrophobic interaction chromatography in protein purification applications / J.T. McCue -- Affinity chromatography: general methods / M. Urh, D. Simpson and K. Zhao -- Immobilized-metal affinity chromatography (IMAC): a review / H. Block ... [et al.] -- Identification, production, and use of polyol-responsive monoclonal antibodies for immunoaffinity chromatography / N.E. Thompson ... [et al.] -- One-dimensional gel electrophoresis / D.E. Garfin -- Isoelectric focusing and two-dimensional gel electrophoresis / D.B. Friedman, S. Hoving and R. Westermeier -- Protein gel staining methods: an introduction and overview / T.H. Steinberg -- Elution of proteins from gels / R.R. Burgess -- Performing and optimizing Western blots with an emphasis on chemiluminescent detection / A. Alegria-Schaffer, A. Lodge and K. Vattem -- Detergents: an overview / D. Linke -- Purification of membrane proteins / S.H. Lin and G. Guidotti -- Purification of recombinant G-protein-coupled receptors / R. Grisshammer -- Cell-free translation of integral membrane proteins into unilamelar liposomes / M.A. Goren ... [et al.] -- Determination of protein purity / D.G. Rhodes and T.M. Laue -- Determination of size, molecular weight, and presence of subunits / D.G. Rhodes, R.E. Bossio and T.M. Laue -- Identification and quantification of protein posttranslational modifications / A.R. Farley and A.J. Link -- Parallel methods for expression and purification / S.A. Lesley -- Techniques to isolate O2-sensitive proteins: [4Fe-4S]-FNR as an example / A. Yan and P.J. Kiley -- Rethinking your purification procedure / M.P. Deutscher -- Important but little known (or forgotten) artifacts in protein biochemistry / R.R. Burgess.
588 ## - SOURCE OF DESCRIPTION NOTE
Source of description note	Description based on print version record.
520 ## - SUMMARY, ETC.
Summary, etc.	The second edition of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology. Assembles chapters on both common and less-common relevant techniques. Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element	Proteins
General subdivision	Purification.
650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element	Proteins
General subdivision	isolation & purification.
650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element	Proteins
General subdivision	Purification.
Source of heading or term	fast
Authority record control number	(OCoLC)fst01079750
655 #4 - INDEX TERM--GENRE/FORM
Genre/form data or focus term	Electronic books.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name	Burgess, Richard R.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name	Deutscher, Murray P.
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information	Print version:
Title	Guide to protein purification.
Edition	2nd ed.
Place, publisher, and date of publication	London : Academic, 2009
International Standard Book Number	9780123745361
Record control number	(OCoLC)423582245
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title	Methods in enzymology ;
Volume number/sequential designation	v. 463.
856 40 - ELECTRONIC LOCATION AND ACCESS
Materials specified	ScienceDirect
Uniform Resource Identifier	<a href="http://www.sciencedirect.com/science/book/9780123745361">http://www.sciencedirect.com/science/book/9780123745361</a>
856 40 - ELECTRONIC LOCATION AND ACCESS
Materials specified	ScienceDirect
Uniform Resource Identifier	<a href="http://www.sciencedirect.com/science/bookseries/00766879/463">http://www.sciencedirect.com/science/bookseries/00766879/463</a>
938 ## -
--	Baker and Taylor
--	BTCP
--	BK0008324600
938 ## -
--	YBP Library Services
--	YANK
--	3017446
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--	Coutts Information Services
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--	15046973
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Koha item type	E(electronic)-Books
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