CDNA preparation and characterization (Record no. 21222)
[ view plain ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 05544cam a2200637Ia 4500 |
| 001 - CONTROL NUMBER | |
| control field | ocm56578168 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OCoLC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20141103172215.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS | |
| fixed length control field | m o d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr |m|n u||||| |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 040927s1999 cauaf obf 001 0 eng |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | |
| Language of cataloging | eng |
| Transcribing agency | AZU |
| Modifying agency | OCLCQ |
| -- | CUSER |
| -- | COF |
| -- | OPELS |
| -- | OCLCQ |
| -- | OPELS |
| -- | OCLCF |
| -- | KUK |
| 019 ## - | |
| -- | 326626500 |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 0121822044 (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9780121822040 (electronic bk.) |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)56578168 |
| Canceled/invalid control number | (OCoLC)326626500 |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QP601 |
| Item number | .M49 vol. 303 |
| 060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER | |
| Classification number | W1 |
| Item number | ME9615K v. 303 1999 |
| 060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER | |
| Classification number | QU 58.5 |
| Item number | C386 1999 |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 572.86 |
| Edition number | 21 |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | TEFA |
| 245 00 - TITLE STATEMENT | |
| Title | CDNA preparation and characterization |
| Medium | [electronic resource] / |
| Statement of responsibility, etc. | edited by Sherman M. Weissman. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc. | San Diego : |
| Name of publisher, distributor, etc. | Academic Press, |
| Date of publication, distribution, etc. | c1999. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource (xxxii, 575 p.) : |
| Other physical details | ill. (some col.) |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in enzymology ; |
| Volume/sequential designation | v. 303 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc | Includes bibliographical references and indexes. |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | cDNA preparation -- Gene identification -- Patterns of mRNA expression -- Functional relationship among cDNA translation products. |
| 588 ## - SOURCE OF DESCRIPTION NOTE | |
| Source of description note | Description based on print version record. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Genomic sequences, now emerging at a rapid rate, are greatly expediting certain aspects of molecular biology. However, in more complex organisms, predicting mRNA structure from genomic sequences can often be difficult. Alternative splicing, the use of alternative promoters, and orphan genes without known analogues can call present difficulties in the predictions of the structure of mRNAs or even in gene detection. Both computational and experimental methods remain useful for recognizing genes and transcript templates, even in sequenced DNA. Methods for producing full-length cDNAs are important for determining the structures of the proteins the mRNA encodes, the positions of promoters, and the considerable regulatory information for translation that may be encoded in the 5' untranslated regions of the mRNA. Methods for studying levels of mRNA and their changes in different physiological circumstances are rapidly evolving, and the information from this area will rival the superabundance of information derived from genomic sequences. In particular, cDNAs can be prepared even from single cells, and this approach has already yielded valuable information in several areas. To the extent that reliable and reproducible information, both quantitative and qualitative, can be generated from very small numbers of cells, there are rather remarkable possibilities for complementing functional and genetic analysis of developmental patterns with descriptions of changes in mRNAs. Dense array analysis promises to be particularly valuable for the rapid expression pattern of known genes, while other methods such as gel display approaches offer the opportunity of discovering unidentified genes or for investigating species whose cDNAs or genomes have not been studied intensively. Knowledge of mRNA structure, genomic location, and patterns of expression must be converted into information of the function of the encoded proteins. Each gene can be the subject of years of intensive study. Nevertheless, a number of methods are being developed that use cDNA to predict properties or permit the selective isolation of cDNAs encoding proteins with certain general properties such as selective isolation of cDNAs encoding proteins with certain general properties such as subcellular location. This volume presents an update of a number of approaches relevant to the areas referred to above. The technology in this field is rapidly evolving and these contributions represent a "snapshot in time" of the number of currently available and useful approaches to the problems referred to above. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA |
| Form subdivision | Handbooks, manuals, etc. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Messenger RNA. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Gene mapping. |
| 650 12 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA, Complementary |
| General subdivision | isolation & purification. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Base Sequence. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Chromosome Mapping |
| General subdivision | methods. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA |
| General subdivision | analysis. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA, Complementary |
| General subdivision | analysis. |
| 650 22 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | RNA, Messenger |
| General subdivision | analysis. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA |
| General subdivision | Analysis. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Nucleotide sequence. |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | ADN |
| General subdivision | Guides, manuels, etc. |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | ARN messager. |
| 650 #6 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cartes chromosomiques. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | DNA. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst00886555 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Gene mapping. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst00939625 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Messenger RNA. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst01017394 |
| 655 #4 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| 655 #7 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Handbooks, manuals, etc. |
| Source of term | fast |
| Authority record control number | (OCoLC)fst01423877 |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Weissman, Sherman M. |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Print version: |
| Title | CDNA preparation and characterization. |
| Place, publisher, and date of publication | San Diego : Academic Press, c1999 |
| International Standard Book Number | 0121822044 |
| Record control number | (OCoLC)41408954 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in enzymology ; |
| Volume number/sequential designation | v. 303. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | ScienceDirect |
| Uniform Resource Identifier | <a href="http://www.sciencedirect.com/science/book/9780121822040">http://www.sciencedirect.com/science/book/9780121822040</a> |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | ScienceDirect |
| Uniform Resource Identifier | <a href="http://www.sciencedirect.com/science/bookseries/00766879/303">http://www.sciencedirect.com/science/bookseries/00766879/303</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | E(electronic)-Books |
| 994 ## - | |
| -- | C0 |
| -- | TEF |
No items available.
