Nuclear pore complexes and nucleocytoplasmic transport - Methods / (Record no. 21085)
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| fixed length control field | 05960cam a2200745Ii 4500 |
| 001 - CONTROL NUMBER | |
| control field | ocn880827118 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OCoLC |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20141103172213.0 |
| 006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS | |
| fixed length control field | m o d |
| 007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION | |
| fixed length control field | cr cnu---unuuu |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 140531t20142014onc o 000 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | |
| Language of cataloging | eng |
| Description conventions | rda |
| -- | pn |
| Transcribing agency | EBLCP |
| Modifying agency | N$T |
| -- | UIU |
| -- | CUS |
| -- | OCLCF |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9780124171787 (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 0124171788 (electronic bk.) |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| Canceled/invalid ISBN | 9780124171602 |
| 029 1# - (OCLC) | |
| OCLC library identifier | DEBSZ |
| System control number | 414275993 |
| 035 ## - SYSTEM CONTROL NUMBER | |
| System control number | (OCoLC)880827118 |
| 050 #4 - LIBRARY OF CONGRESS CALL NUMBER | |
| Classification number | QH595 |
| Item number | .N384 2014eb |
| 072 #7 - SUBJECT CATEGORY CODE | |
| Subject category code | SCI |
| Subject category code subdivision | 008000 |
| Source | bisacsh |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Classification number | 574.87/32 |
| Edition number | 23 |
| 049 ## - LOCAL HOLDINGS (OCLC) | |
| Holding library | TEFA |
| 245 00 - TITLE STATEMENT | |
| Title | Nuclear pore complexes and nucleocytoplasmic transport - Methods / |
| Statement of responsibility, etc. | edited by Val�erie Doye. |
| 250 ## - EDITION STATEMENT | |
| Edition statement | First edition. |
| 264 #1 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Place of production, publication, distribution, manufacture | San Diego, CA : |
| Name of producer, publisher, distributor, manufacturer | Academic Press, |
| Date of production, publication, distribution, manufacture, or copyright notice | [2014]. |
| 264 #4 - PRODUCTION, PUBLICATION, DISTRIBUTION, MANUFACTURE, AND COPYRIGHT NOTICE | |
| Date of production, publication, distribution, manufacture, or copyright notice | �2014 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 1 online resource (xx, 508 pages) : |
| Other physical details | color illustrations. |
| 336 ## - CONTENT TYPE | |
| Content type term | text |
| Content type code | txt |
| Source | rdacontent |
| 337 ## - MEDIA TYPE | |
| Media type term | computer |
| Media type code | c |
| Source | rdamedia |
| 338 ## - CARRIER TYPE | |
| Carrier type term | online resource |
| Carrier type code | cr |
| Source | rdacarrier |
| 490 1# - SERIES STATEMENT | |
| Series statement | Methods in Cell Biology ; |
| Volume/sequential designation | volume 122 |
| 500 ## - GENERAL NOTE | |
| General note | Description based upon print version of record. |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Front Cover; Nuclear Pore Complexes and Nucleocytoplasmic Transport -- Methods; Copyright; Contents; Contributors; Preface; Chapter 1: Fifty Years of Nuclear Pores and Nucleocytoplasmic Transport Studies: Multiple Tools Revealing Complex Rules; Introduction; 1.1. The NPCs: A Modular Macromolecular Assembly; 1.1.1. Toward a refined view of the NPC structure; 1.1.2. Nucleoporins: The building blocks of NPCs; 1.1.3. Integrating the Nups into the 3D architecture of the NPC; 1.1.3.1. Nup localization; 1.1.3.2. Interactions among Nups; 1.1.3.3. Nup stoichiometry |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 1.1.3.4. Toward a detailed map of NPCs1.1.4. Nups are composed of a limited set of structural domains; 1.2. Nucleocytoplasmic Trafficking: The Rules of the Road; 1.2.1. Investigating nucleocytoplasmic transport; 1.2.1.1. Historical overview; 1.2.1.2. Expanding the toolbox; 1.2.2. The signals for nucleocytoplasmic exchanges; 1.2.3. A family of protein transport receptors: The karyopherins; 1.2.4. The Ran GTPase: A key to transport directionality; 1.2.5. The case of INM targeting; 1.2.6. Distinct pathways contribute to RNA export |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 1.2.7. Translocation across the NPCs: A dual function for FG-Nups as barrier and gate1.2.8. Noncanonical transport pathways through the NE; 1.3. The Nuclear Transport Machinery: A Dynamic and Versatile Device; 1.3.1. NPC biogenesis throughout the cell cycle; 1.3.1.1. NPC disassembly; 1.3.1.2. Post-mitotic NPC assembly; 1.3.1.3. De novo NPC assembly; 1.3.2. Multiple functions of the nuclear transport machinery during the cell cycle; 1.3.3. NPCs, nuclear organization, and gene expression; 1.3.4. NPCs and genetic stability; Concluding Remarks; Acknowledgments; References |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Chapter 2: Imaging Metazoan Nuclear Pore Complexes by Field Emission Scanning Electron MicroscopyIntroduction; Prehistoric landmarks (before EM): From cells to the nuclear envelope; The EM era: From the nuclear envelope to nuclear pore complexes; High resolution SEM: Direct surface imaging of NPCs; 2.1. Rationale; 2.1.1. Purpose; 2.1.2. An overview of sample preparation; 2.1.2.1. Fixation; 2.1.2.2. Dehydration and critical point drying; 2.1.2.3. Sputter coating; 2.2. Materials; 2.2.1. Equipment; 2.2.2. Materials; 2.2.3. Reagents; 2.2.4. Buffers; 2.3. Anchored Nuclei |
| 505 8# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | 2.3.1. Coating silicon chips2.3.2. Chromatin decondensation and attachment; 2.3.3. In vitro assembly reaction; 2.4. Mammalian Cell Nuclei; 2.5. Immunogold Labeling; 2.6. Sample Preparation for FESEM; 2.6.1. Fixation, dehydration, and CPD; 2.6.2. Sputter coating and FESEM analysis; Acknowledgments; References; Chapter 3: Imaging Yeast NPCs: From Classical Electron Microscopy to Immuno-SEM; Introduction; 3.1. Conventional TEM; 3.1.1. Materials; 3.1.1.1. Equipment; 3.1.1.2. Spheroplast preparation; 3.1.1.3. Fixation, dehydration, contrasting, and embedding; 3.1.1.4. Sectioning and poststaining |
| 500 ## - GENERAL NOTE | |
| General note | 3.1.2. Procedure |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Volume 122 of Methods in Cell Biology describes modern tools and techniques used to study nuclear pore complexes and nucleocytoplasmic transport in diverse eukaryotic model systems (including mammalian cells, Xenopus, C. elegans, yeast). The volume enables investigators to analyze nuclear pore complex structure, assembly, and dynamics; to evaluate protein and RNA trafficking through the nuclear envelope; and to design in vivo or in vitro assays appropriate to their research needs. Beyond the study of nuclear pores and transport as such, these protocols will also. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cell nuclei. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Nuclear membranes. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cytoplasm. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Biological transport. |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cell Nucleus. |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Nuclear Pore. |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Active Transport, Cell Nucleus. |
| 650 #2 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cytological Techniques. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cell biology. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cell nuclei. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Science. |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | SCIENCE / Life Sciences / Biology |
| Source of heading or term | bisacsh |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Biological transport. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst00832345 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cell nuclei. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst00850208 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Cytoplasm. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst00886318 |
| 650 #7 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Nuclear membranes. |
| Source of heading or term | fast |
| Authority record control number | (OCoLC)fst01040367 |
| 655 #4 - INDEX TERM--GENRE/FORM | |
| Genre/form data or focus term | Electronic books. |
| 700 1# - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Doye, Val�erie, |
| Relator term | editor. |
| 776 08 - ADDITIONAL PHYSICAL FORM ENTRY | |
| Relationship information | Print version: |
| Main entry heading | Doye, Val�erie. |
| Title | Nuclear pore complexes and nucleocytoplasmic transport - Methods. |
| Place, publisher, and date of publication | Burlington : Elsevier Science, 2014 |
| International Standard Book Number | 9780124171787 |
| 830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE | |
| Uniform title | Methods in cell biology ; |
| Volume number/sequential designation | v. 122. |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Materials specified | ScienceDirect |
| Uniform Resource Identifier | <a href="http://www.sciencedirect.com/science/book/9780124171602">http://www.sciencedirect.com/science/book/9780124171602</a> |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://www.sciencedirect.com/science/publication?issn=0091679X&volume=122">http://www.sciencedirect.com/science/publication?issn=0091679X&volume=122</a> |
| 938 ## - | |
| -- | EBL - Ebook Library |
| -- | EBLB |
| -- | EBL1694271 |
| 938 ## - | |
| -- | EBSCOhost |
| -- | EBSC |
| -- | 784139 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | E(electronic)-Books |
| 994 ## - | |
| -- | C0 |
| -- | TEF |
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