Electron microscopy : methods and protocols
Electron microscopy : methods and protocols
- 3rd edition.
- New York : Humana Press, 2014.
- xviii, 799 pages : illustrations ; 26 cm
- Methods in molecular biology, 1117 1064-3745 ; Springer protocols .
Preceded by: Electron microscopy / edited by John Kuo. 2nd ed. c2007.
Includes bibliographical references and index.
This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.
9781627037754
Electron microscopy--Laboratory manuals.
Histology--Technique.
Microscopy, Electron--Laboratory Manuals.
Histocytological Preparation Techniques--Laboratory Manuals.
DNA and RNA tracking
Scanning electron microscope (SEM)
Bio-molecular research
Correlative microscopy
cryoTEM 3D tomography
Laboratory Manuals.
57.086.3 / KUO-E
Preceded by: Electron microscopy / edited by John Kuo. 2nd ed. c2007.
Includes bibliographical references and index.
This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.
9781627037754
Electron microscopy--Laboratory manuals.
Histology--Technique.
Microscopy, Electron--Laboratory Manuals.
Histocytological Preparation Techniques--Laboratory Manuals.
DNA and RNA tracking
Scanning electron microscope (SEM)
Bio-molecular research
Correlative microscopy
cryoTEM 3D tomography
Laboratory Manuals.
57.086.3 / KUO-E
