MicroRNA methods
MicroRNA methods [electronic resource] /
edited by John J. Rossi, Gregory J. Hannon.
- San Diego, Calif. : Academic Press, c2007.
- 1 online resource (xl, 261 p., [8] p. of plates) : ill. (some col.)
- Methods in enzymology, v. 427 0076-6879 ; .
- Methods in enzymology ; v. 427. .
Includes bibliographical references and indexes.
Identification of viral microRNAs / Robust machine learning algorithms predict microRNA genes and targets / Idenfication of virally encoded microRNAs / Computational methods for microRNA target prediction / In vitro and in vivo assays for the activity of Drosha complex / Microarray analysis of miRNA gene expression / Cloning and detecting signature microRNAs from mammalian cells / Christopher S. Sullivan and Adam Grundhoff -- Pal Saetrom and Ola Snove, Jr. -- Sebastien Pfeffer -- Yuka Watanabe, Masaru Tomita, and Akio Kanai -- Yoontae Lee and V. Narry Kim -- J. Michael Thomson, Joel S. Parker, and Scott M. Hammond -- Guihua Sun, Haitang Lik and Gohn J. Rossi. Ch. 1. Ch. 2. Ch. 3. Ch. 4. Ch. 5. Ch. 6. Ch. 7. Approaches for studying microRNA and small interfering RNA methylation in vitro and in vivo / Analysis of small RNA profiles during development / Dissecting microRNA-mediated gene regulation and function in T cell development / Investigation of microRNAs alterations in leukemias and lymphomas / Discovery of pathogen-regulated small RNAs in plants / Protocols for expression and functional analysis of viral microRNAs / Zhiyong Yang ... [et al.] -- Toshiaki Watanabe ... [et al.] -- Tin Ky Mao and Chang-Zheng Chen -- George Adrian Calin and Carlo Maria Croce -- Surekha Katiyar-Agarwal and Hailing Jin -- Eva Gottwein and Bryan R. Cullen. Ch. 8. Ch. 9. Ch. 10. Ch. 11. Ch. 12. Ch. 13.
MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; Micro RNA expression, maturation and functional analysis; and MicroRNAs and disease.
9780080551395 (electronic bk.) 0080551394 (electronic bk.) 0123739179 (electronic bk.) 9780123739179 (electronic bk.)
Small interfering RNA.
SCIENCE--Life Sciences--Molecular Biology.
Small interfering RNA.
MicroRNAs.
RNA, Small Interfering.
Electronic books.
QP601 / .C733 2007eb
572.88
QU 58.7 / M6258 2007
Includes bibliographical references and indexes.
Identification of viral microRNAs / Robust machine learning algorithms predict microRNA genes and targets / Idenfication of virally encoded microRNAs / Computational methods for microRNA target prediction / In vitro and in vivo assays for the activity of Drosha complex / Microarray analysis of miRNA gene expression / Cloning and detecting signature microRNAs from mammalian cells / Christopher S. Sullivan and Adam Grundhoff -- Pal Saetrom and Ola Snove, Jr. -- Sebastien Pfeffer -- Yuka Watanabe, Masaru Tomita, and Akio Kanai -- Yoontae Lee and V. Narry Kim -- J. Michael Thomson, Joel S. Parker, and Scott M. Hammond -- Guihua Sun, Haitang Lik and Gohn J. Rossi. Ch. 1. Ch. 2. Ch. 3. Ch. 4. Ch. 5. Ch. 6. Ch. 7. Approaches for studying microRNA and small interfering RNA methylation in vitro and in vivo / Analysis of small RNA profiles during development / Dissecting microRNA-mediated gene regulation and function in T cell development / Investigation of microRNAs alterations in leukemias and lymphomas / Discovery of pathogen-regulated small RNAs in plants / Protocols for expression and functional analysis of viral microRNAs / Zhiyong Yang ... [et al.] -- Toshiaki Watanabe ... [et al.] -- Tin Ky Mao and Chang-Zheng Chen -- George Adrian Calin and Carlo Maria Croce -- Surekha Katiyar-Agarwal and Hailing Jin -- Eva Gottwein and Bryan R. Cullen. Ch. 8. Ch. 9. Ch. 10. Ch. 11. Ch. 12. Ch. 13.
MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; Micro RNA expression, maturation and functional analysis; and MicroRNAs and disease.
9780080551395 (electronic bk.) 0080551394 (electronic bk.) 0123739179 (electronic bk.) 9780123739179 (electronic bk.)
Small interfering RNA.
SCIENCE--Life Sciences--Molecular Biology.
Small interfering RNA.
MicroRNAs.
RNA, Small Interfering.
Electronic books.
QP601 / .C733 2007eb
572.88
QU 58.7 / M6258 2007
