Microbial metagenomics, metatranscriptomics, and metaproteomics /
Microbial metagenomics, metatranscriptomics, and metaproteomics /
edited by Edward F. DeLong, Morton and Claire Goulder Family, Professor in Environmental Systems, Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, Dept. of Biological Engineering, Cambridge, MA, USA and Center for Microbial Ecology, Research and Education, University of Hawaii, Manoa Honolulu, HI.
- First edition.
- 1 online resource (lix, 598 pages, 24 unnumbered pages of plates) : illustrations (some color).
- Methods in enzymology, volume 531 0076-6879 ; .
- Methods in enzymology ; v. 531. .
Includes bibliographical references and indexes.
In-solution fluorescence in situ hybridization and fluorescence-activated cell sorting for single cell and population genome recovery / Whole cell immunomagnetic enrichment of environmental microbial consortia using rRNA-targeted magneto-FISH / Coupling FACS and genomic methods for the characterization of uncultivated symbionts / Optofluidic cell selection from complex microbial communities for single-genome analysis / Quantifying and identifying the active and damaged subsets of indigenous microbial communities / Preparation of BAC libraries from marine microbial populations / Preparation of fosmid libraries and functional metagenomic analysis of microbial community DNA / Preparation of metagenomic libraries from naturally occurring marine viruses / Preparation and metatranscriptomic analyses of host-microbe systems / Preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton / Sequential isolation of metabolites, RNA, DNA, and proteins from the same unique sample / Use of internal standards for quantitative metranscriptome and metagenome analysis / Sample processing and cDNA preparation for microbial metatranscriptomics in complex soil communities / Sample preparation and processing for planktonic microbial community proteomics / Sample handling and mass spectrometry for microbial metaproteomic analyses / Molecular tools for investigating mcirobial community structure and function in oxygen-deficient marine waters / Assembling full-length rRNA genes from short-read metagenomic sequence datasets using EMIRGE / Computational methods for high-throughput comparative analyses of natural microbial communities / Advancing our understanding of the human microbiome using QIIME / Disentangling associated genomes / Microbial community analysis using MEGAN / A metagenomics portal for a democratized sequencing world / A user's guide to quantitative and comparative analysis of metagenomic datasets / Mohamed F. Haroon, Connor T. Skennerton, Jason A. Steen, Nancy Lachner, Philip Hugenholtz, and Gene W. Tyson -- Elizabeth Trembath-Reichert, Abigail Green-Saxena, and Victoria J. Orphan -- Anne Thompson, Shellie Bench, Brandon Carter, and Jonathan Zehr -- Zachary C. Landry, Stephen J. Giovanonni, Stephen R. Quake, and Paul C. Blainey -- Corinne Ferrier Maurice and Peter James Turnbaugh -- Gazalah Sabehi and Oded B�ej�a -- Asunci�on Mart�inez and Marcia S. Osburne -- Sergei A. Solonenko and Matthew B. Sullivan -- Jarrad T. Hampton-Marcell, Stephanie M. Moormann, Sarah M. Owens, and Jack A. Gilbert -- Frank J. Stewart -- Hugo Roume, Anna Heintz-Buschart, Emilie EL Muller, and Paul Wilmes -- Brandon M. Satinsky, Scott M. Gilford, Byron C. Crump, and Mary Ann Moran -- Lilia C. Carvalhais and Peer M. Schenk -- Robert M. Morris and Brook L. Nunn -- Ryan S. Mueller and Chongle Pan -- Alyse K. Hawley, Sam Kheirandish, Andreas Mueller, Hilary T.C. Leung, Angela D. Norbeck, Heather M. Brewer, Ljiljana Pasa-Tolic, and Steven J. Hallam -- Christopher S. Miller -- Sarah P. Preheim, Allison R. Perrotta, Jonathan Friedman, Chris Smilie, Ilana Brito, Mark B. Smith, and Eric Alm -- Jos�e A. Navas-Molina, Juan M. Peralta-S�anchez, Antonio Gonz�alez, Paul J. McMurdie, Yoshiki V�azquez-Baeza, Zhenjiang Xu, Luke K. Ursell, Christian Lauber, Hongwei Zhou, Se Jin Song, James Huntley, Gail L. Ackermann, Donna Berg-Lyons, Susan Holmes, J. Gregory Caporaso, and Rob Knight -- Daniel B. Sloan, Gordon M. Bennett, Philipp Engel, David Williams, and Howard Ochman -- Daniel H. Huson and Nico Weber -- Andreas Wilke, Elizabeth M. Glass, Daniela Bartels, Jared Bischof, Daniel Braithwaite, Mark D'Souza, Wolfgang Gerlach, Travis Harrison, Kevin Keegan, Hunter Matthews, Renzo Kottmann, Tobias Paczian, Wei Tang, William L. Trimble, Pelin Yilmaz, Jared Wilkening, Narayan Desai, and Folker Meyer -- Chengwei Luo, Luis M. Rodriguez-R, and Konstantinos T. Konstantinidis.
9780124079274 (electronic bk.) 012407927X (electronic bk.)
Microbial genomics.
Proteomics.
RNA--Research--Methodology.
Enzymes.
Biochemistry--Technique.
Microorganisms.
Metagenomics.
Metagenomics--methods.
SCIENCE / Life Sciences / Biochemistry
Electronic books.
QP601 / .M48 2013eb
572.8629
Includes bibliographical references and indexes.
In-solution fluorescence in situ hybridization and fluorescence-activated cell sorting for single cell and population genome recovery / Whole cell immunomagnetic enrichment of environmental microbial consortia using rRNA-targeted magneto-FISH / Coupling FACS and genomic methods for the characterization of uncultivated symbionts / Optofluidic cell selection from complex microbial communities for single-genome analysis / Quantifying and identifying the active and damaged subsets of indigenous microbial communities / Preparation of BAC libraries from marine microbial populations / Preparation of fosmid libraries and functional metagenomic analysis of microbial community DNA / Preparation of metagenomic libraries from naturally occurring marine viruses / Preparation and metatranscriptomic analyses of host-microbe systems / Preparation of microbial community cDNA for metatranscriptomic analysis in marine plankton / Sequential isolation of metabolites, RNA, DNA, and proteins from the same unique sample / Use of internal standards for quantitative metranscriptome and metagenome analysis / Sample processing and cDNA preparation for microbial metatranscriptomics in complex soil communities / Sample preparation and processing for planktonic microbial community proteomics / Sample handling and mass spectrometry for microbial metaproteomic analyses / Molecular tools for investigating mcirobial community structure and function in oxygen-deficient marine waters / Assembling full-length rRNA genes from short-read metagenomic sequence datasets using EMIRGE / Computational methods for high-throughput comparative analyses of natural microbial communities / Advancing our understanding of the human microbiome using QIIME / Disentangling associated genomes / Microbial community analysis using MEGAN / A metagenomics portal for a democratized sequencing world / A user's guide to quantitative and comparative analysis of metagenomic datasets / Mohamed F. Haroon, Connor T. Skennerton, Jason A. Steen, Nancy Lachner, Philip Hugenholtz, and Gene W. Tyson -- Elizabeth Trembath-Reichert, Abigail Green-Saxena, and Victoria J. Orphan -- Anne Thompson, Shellie Bench, Brandon Carter, and Jonathan Zehr -- Zachary C. Landry, Stephen J. Giovanonni, Stephen R. Quake, and Paul C. Blainey -- Corinne Ferrier Maurice and Peter James Turnbaugh -- Gazalah Sabehi and Oded B�ej�a -- Asunci�on Mart�inez and Marcia S. Osburne -- Sergei A. Solonenko and Matthew B. Sullivan -- Jarrad T. Hampton-Marcell, Stephanie M. Moormann, Sarah M. Owens, and Jack A. Gilbert -- Frank J. Stewart -- Hugo Roume, Anna Heintz-Buschart, Emilie EL Muller, and Paul Wilmes -- Brandon M. Satinsky, Scott M. Gilford, Byron C. Crump, and Mary Ann Moran -- Lilia C. Carvalhais and Peer M. Schenk -- Robert M. Morris and Brook L. Nunn -- Ryan S. Mueller and Chongle Pan -- Alyse K. Hawley, Sam Kheirandish, Andreas Mueller, Hilary T.C. Leung, Angela D. Norbeck, Heather M. Brewer, Ljiljana Pasa-Tolic, and Steven J. Hallam -- Christopher S. Miller -- Sarah P. Preheim, Allison R. Perrotta, Jonathan Friedman, Chris Smilie, Ilana Brito, Mark B. Smith, and Eric Alm -- Jos�e A. Navas-Molina, Juan M. Peralta-S�anchez, Antonio Gonz�alez, Paul J. McMurdie, Yoshiki V�azquez-Baeza, Zhenjiang Xu, Luke K. Ursell, Christian Lauber, Hongwei Zhou, Se Jin Song, James Huntley, Gail L. Ackermann, Donna Berg-Lyons, Susan Holmes, J. Gregory Caporaso, and Rob Knight -- Daniel B. Sloan, Gordon M. Bennett, Philipp Engel, David Williams, and Howard Ochman -- Daniel H. Huson and Nico Weber -- Andreas Wilke, Elizabeth M. Glass, Daniela Bartels, Jared Bischof, Daniel Braithwaite, Mark D'Souza, Wolfgang Gerlach, Travis Harrison, Kevin Keegan, Hunter Matthews, Renzo Kottmann, Tobias Paczian, Wei Tang, William L. Trimble, Pelin Yilmaz, Jared Wilkening, Narayan Desai, and Folker Meyer -- Chengwei Luo, Luis M. Rodriguez-R, and Konstantinos T. Konstantinidis.
9780124079274 (electronic bk.) 012407927X (electronic bk.)
Microbial genomics.
Proteomics.
RNA--Research--Methodology.
Enzymes.
Biochemistry--Technique.
Microorganisms.
Metagenomics.
Metagenomics--methods.
SCIENCE / Life Sciences / Biochemistry
Electronic books.
QP601 / .M48 2013eb
572.8629
